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M94A2697.TXT
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1994-10-25
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Document 2697
DOCN M94A2697
TI Evaluation of Cambridge Biotech western blot kit.
DT 9412
AU Senyonga P; Kataaha; Nakasero Blood Bank, Kampala-Uganda.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):245 (abstract no. PB0408). Unique
Identifier : AIDSLINE ICA10/94369878
AB OBJECTIVES: To assess the suitability of the kit for HIV1 + 2
confirmation. MATERIALS: The kit was submitted for evaluation by the
company representative, clearly labelled with: the manufacturers
address, to be used for HIV1 + 2 testing, contents and quantities,
storage temperature and expiry date. 26 frozen, Elisa tested and Inno
Lia blotted samples were tested. METHODS: Manufacturers recommended
method was used to test the samples and the 4 controls supplied by the
company. RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME. RESULTS ANALYSIS:
In addition to P24, cambridge Biotech detected ENV. 160 probably early
sero conversion in the two samples. DISCUSSION: Clear instructions and
easy to carry out, specificity and sensitivity comparable to that of
Innolia. The detection of ENV. 160/120 is an added advantage, indicative
of early sero-conversion.
DE *AIDS Serodiagnosis *Blotting, Western Comparative Study Gene
Products, env/IMMUNOLOGY Human HIV Core Protein p24/IMMUNOLOGY HIV
Seropositivity/*DIAGNOSIS/IMMUNOLOGY HIV-1/*IMMUNOLOGY
HIV-2/*IMMUNOLOGY Predictive Value of Tests Protein
Precursors/IMMUNOLOGY Reagent Kits, Diagnostic MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).